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KMID : 0359319960360030647
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Korean Journal of Veterinary Research
1996 Volume.36 No. 3 p.647 ~ p.655
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Characterization of immunosuppressive factors in the mastitis - infected mammary gland of non - lactating cows
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Abstract
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To establish the effective ways to prevent bovine mastitis, the study has been performed to investigate the attributable factors causing down-regulation of immune responses in mammary gland of non-lactating cows.
Lymphocytes from peripheral blood and mammary gland secretions(MGS) were obtained from normal healthy cows and mastitic cows, respectively. Lymphoblastogenesis were investigated carefully by adding different concentrations of supernatants collected from purecultures of neutrophils seperated from peripheral blood and MGS, respectively.
The results obtained are as follows ;
1. Lymphoblastogenesis activity stimulated by Con A, PWM and PHA were significantly reduced in MGS from mastitic cows.
2. Supernatants collected from pure-culture of neutrophils separated both from peripheral blood and MGS showed inhibitory effect on mitogenic lymphoblastogenesis.
3. Supernatants from mammary gland neutrophils have shown 7 times more inhibitory activity than those from peripheral blood and this inhibitory effect was increased in proportion to increasing concentrations of supernatants when those were added to lymphoblast cells in culture.
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